Difference between revisions of "Part:BBa K3110005"
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<partinfo>BBa_K3110005 short</partinfo> | <partinfo>BBa_K3110005 short</partinfo> | ||
| − | L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a medium RBS. | + | This construct has L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a medium RBS. |
lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various lower and higher concentrations of lldD by varying the strength of the promoter and RBS controlling its production. | lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various lower and higher concentrations of lldD by varying the strength of the promoter and RBS controlling its production. | ||
Revision as of 19:31, 18 October 2019
Strong Promoter Medium RBS lldD
This construct has L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a medium RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various lower and higher concentrations of lldD by varying the strength of the promoter and RBS controlling its production.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1203
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 640