Difference between revisions of "Part:BBa K2984021"
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[[Image:Terminator.png]]
 
 
<partinfo>BBa_K2984021 parameters</partinfo>
 
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<partinfo>BBa_K2984021 short</partinfo>
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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. To terminate the transcription of a gene, a signal on the DNA is required. The nucleotide sequence mediating this signal to the RNA-polymerase is called terminator. In 1986 a Chlamydomonas reinhardtii native gene for the small subunits of the ribulose bisphosphate carboxylase/oxygenase (Rubisco) was characterized (Goldschmidt-Clermont and Rahire, 1968; Stevens et al., 1996).
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* This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. To terminate the transcription of a gene, a signal on the DNA is required. The nucleotide sequence mediating this signal to the RNA-polymerase is called terminator. In 1986 a Chlamydomonas reinhardtii native gene for the small subunits of the ribulose bisphosphate carboxylase/oxygenase (Rubisco) was characterized (Goldschmidt-Clermont and Rahire, 1968; Stevens et al., 1996).
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'''Secondary Structure'''
 
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[[Image:Mfold-K2984021-1.png]]
 
  
 
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<partinfo>BBa_K2984021 SequenceAndFeatures</partinfo>
 
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'''Measurement'''
 
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
 
  
 
==References==
 
==References==

Revision as of 19:17, 18 October 2019



This part is a part of the Chlamy-HUB-Collection. To terminate the transcription of a gene, a signal on the DNA is required. The nucleotide sequence mediating this signal to the RNA-polymerase is called terminator. In 1986 a Chlamydomonas reinhardtii native gene for the small subunits of the ribulose bisphosphate carboxylase/oxygenase (Rubisco) was characterized (Goldschmidt-Clermont and Rahire, 1968; Stevens et al., 1996).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Goldschmidt-Clermont, M., & Rahire, M. (1986). Sequence, evolution and differential expression of the two genes encoding variant small subunits of ribulose bisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Journal of molecular biology, 191(3), 421-432.