Difference between revisions of "Part:BBa K3110004"

Revision as of 17:49, 18 October 2019

Strong Promoter Strong RBS lldD Terminator

L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a strong RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1202
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 639

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	L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a strong RBS.		L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a strong RBS.
−	lldD cleaves L-lactate to pyruvate~~. Strong promoter and strong RBS ensures high rate of lldD production~~. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production.	+	lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various lower concentrations of lldD by varying the strength of the promoter and RBS controlling its production.