Difference between revisions of "Part:BBa K3105676:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Functionality in Pichia pastoris
+
Functionality in Pichia pastoris <br>
 +
A double stop codon is introduced in the 5'end to stop protein translation. Residues of the pPICZ&#945;B vector are present before the actual terminator starts.
  
  
Line 13: Line 14:
 
===Source===
 
===Source===
  
−
Pichia pastoris, part was taken from pPICZ&#945;B vector from Invitrogen easy select Pichia expression kit  
+
Organism : <I>Pichia pastoris</I> <br>
 +
DNA originates from the pPICZ&#945;B vector found in the invitrogen easy select <I>Pichia</I> expression kit
  
 
===References===
 
===References===

Revision as of 17:36, 18 October 2019


pPICZαB fragment + AOX1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 131
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Functionality in Pichia pastoris
A double stop codon is introduced in the 5'end to stop protein translation. Residues of the pPICZαB vector are present before the actual terminator starts.


Source

Organism : Pichia pastoris
DNA originates from the pPICZαB vector found in the invitrogen easy select Pichia expression kit

References