This part has a GFP spaceholder for the gRNA of the crRNA of the CRISPR/cpf1 system. It is used to insert a gRNA for targeted knockouts in the CRISPR/cpf1 system of <a href="https://2019.igem.org/Team:Marburg"> iGEM Marburg 2019.</a>
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===Compability===
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These parts are compatible with the RCF [1000] standard. These parts are compatible with any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design">Design of the Marburg Collection iGEM Marburg 2018</a>.
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Latest revision as of 17:23, 18 October 2019
pMC_0_3-5_1_crRNA-GFP_dropout
Sequence and Features
Assembly Compatibility:
10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1186
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1186
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1186
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 242
Description
This part has a GFP spaceholder for the gRNA of the crRNA of the CRISPR/cpf1 system. It is used to insert a gRNA for targeted knockouts in the CRISPR/cpf1 system of iGEM Marburg 2019.
Compability
These parts are compatible with the RCF [1000] standard. These parts are compatible with any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection iGEM Marburg 2018.
