Difference between revisions of "Part:BBa K2963014"

 
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<partinfo>BBa_K2963014 short</partinfo>
 
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According to that pre-cistron can affect the expression of next cistron, and we select a short 5' sequence of high-protein-abundance gene from Corynebacterium glutamicum as the pre-cistron trying to improve the stabilization of the expression of racE(coding racemase enzyme).
 
  
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===Usage and Biology===
 
===Usage and Biology===
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The bicistronic has a translational coupling effect. Using bicistronics can stabilize the expression of the target gene.In order to more precisely regulate the activity of the racE gene, our team worked with the master in our lab to further stabilize the expression of the racE gene by using the bicistronic system.
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Revision as of 16:03, 18 October 2019


BsracE18 - Bicistronic containing RBS18 to stabilize gene expression.


Usage and Biology

The bicistronic has a translational coupling effect. Using bicistronics can stabilize the expression of the target gene.In order to more precisely regulate the activity of the racE gene, our team worked with the master in our lab to further stabilize the expression of the racE gene by using the bicistronic system.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 132
    Illegal NheI site found at 999
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 132
  • 1000
    COMPATIBLE WITH RFC[1000]