Difference between revisions of "Part:BBa K2963039:Design"

 
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===Design Notes===
 
===Design Notes===
  
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  The construction methods and experiment steps please visit our experiment page
  
  
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===References===
 
===References===
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1. Xu P, Vansiri A, Bhan N, et al. ePathBrick: a synthetic biology platform for engineering metabolic pathways in E. coli[J]. ACS Synthetic Biology, 2012, 1(7): 256-266.
 +
 +
2.Mutalik, Vivek K, et al. "Precise and reliable gene expression via standard transcription and translation initiation elements." Nature Methods 10.4(2013):354.

Revision as of 15:51, 18 October 2019


Producing γ-PGA with different D/L glutamate monomer ratio(R).


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
    Illegal NheI site found at 2365
    Illegal NheI site found at 2963
    Illegal NheI site found at 4281
    Illegal NheI site found at 5248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1912
    Illegal EcoRI site found at 2756
    Illegal EcoRI site found at 2815
    Illegal NgoMIV site found at 2590
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction methods and experiment steps please visit our experiment page


Source

References

1. Xu P, Vansiri A, Bhan N, et al. ePathBrick: a synthetic biology platform for engineering metabolic pathways in E. coli[J]. ACS Synthetic Biology, 2012, 1(7): 256-266.

2.Mutalik, Vivek K, et al. "Precise and reliable gene expression via standard transcription and translation initiation elements." Nature Methods 10.4(2013):354.