Difference between revisions of "Part:BBa K3190101:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The gene encoding for the receptor was codon optimised and coupled to the strongest constitutive promoter pCCW12 for heterologous expression in S. cerevisiae.  
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The gene encoding for the receptor was codon optimised and coupled to the strongest constitutive promoter pCCW12 (<partinfo>BBa_K3190002</partinfo>) for heterologous expression in S. cerevisiae.  
  
  
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===References===
 
===References===
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<ul>
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  <li>Revankar, C. M. (2005). A Transmembrane Intracellular Estrogen Receptor Mediates Rapid Cell Signaling. <i>Science, 307</i>(5715), 1625–1630. doi: 10.1126/science.1106943</li>
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  <li>Ncbi.nlm.nih.gov. (2019). G-protein coupled estrogen receptor 1 [Homo sapiens] - Protein - NCBI Reference Sequence: NP_001091671.1. [online] Available at: https://www.ncbi.nlm.nih.gov/protein/NP_001091671.1?report=genpept [Accessed 18 Oct. 2019]. </li>
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  <li>Uniprot.org. (2019). GPER1 - G-protein coupled estrogen receptor 1 - Homo sapiens (Human) - GPER1 gene & protein. [online] Available at: https://www.uniprot.org/uniprot/Q99527 [Accessed 18 Oct. 2019].</li>
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</ul>

Latest revision as of 15:36, 18 October 2019


G protein-coupled estrogen receptor (GPER/GPR30) CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 750


Design Notes

The gene encoding for the receptor was codon optimised and coupled to the strongest constitutive promoter pCCW12 (BBa_K3190002) for heterologous expression in S. cerevisiae.


Source

Homo sapiens

References

  • Revankar, C. M. (2005). A Transmembrane Intracellular Estrogen Receptor Mediates Rapid Cell Signaling. Science, 307(5715), 1625–1630. doi: 10.1126/science.1106943
  • Ncbi.nlm.nih.gov. (2019). G-protein coupled estrogen receptor 1 [Homo sapiens] - Protein - NCBI Reference Sequence: NP_001091671.1. [online] Available at: https://www.ncbi.nlm.nih.gov/protein/NP_001091671.1?report=genpept [Accessed 18 Oct. 2019].
  • Uniprot.org. (2019). GPER1 - G-protein coupled estrogen receptor 1 - Homo sapiens (Human) - GPER1 gene & protein. [online] Available at: https://www.uniprot.org/uniprot/Q99527 [Accessed 18 Oct. 2019].