Difference between revisions of "Part:BBa K3190101:Design"

Revision as of 15:34, 18 October 2019

G protein-coupled estrogen receptor (GPER/GPR30) CDS

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 750

Design Notes

The gene encoding for the receptor was codon optimised and coupled to the strongest constitutive promoter pCCW12 (BBa_K3190002) for heterologous expression in S. cerevisiae.

Source

Homo sapiens

References

Revankar, C. M. (2005). A Transmembrane Intracellular Estrogen Receptor Mediates Rapid Cell Signaling. Science, 307(5715), 1625–1630. doi: 10.1126/science.1106943
Ncbi.nlm.nih.gov. (2019). G-protein coupled estrogen receptor 1 [Homo sapiens] - Protein - NCBI Reference Sequence: NP_001091671.1. [online] Available at: https://www.ncbi.nlm.nih.gov/protein/NP_001091671.1?report=genpept [Accessed 18 Oct. 2019].
Reference 3

@@ Line 19: / Line 19: @@
 <ul>
    <li>Revankar, C. M. (2005). A Transmembrane Intracellular Estrogen Receptor Mediates Rapid Cell Signaling. <i>Science, 307</i>(5715), 1625–1630. doi: 10.1126/science.1106943</li>
-   <li>Ncbi.nlm.nih.gov. (2019). G-protein coupled estrogen receptor 1 [Homo sapiens] - Protein - NCBI. [online] Available at: https://www.ncbi.nlm.nih.gov/protein/NP_001091671.1?report=genpept [Accessed 18 Oct. 2019]. NCBI Reference Sequence: NP_001091671.1 </li>
+   <li>Ncbi.nlm.nih.gov. (2019). G-protein coupled estrogen receptor 1 [Homo sapiens] - Protein - NCBI Reference Sequence: NP_001091671.1. [online] Available at: https://www.ncbi.nlm.nih.gov/protein/NP_001091671.1?report=genpept [Accessed 18 Oct. 2019]. </li>
    <li>Reference 3</li>
 </ul>