Difference between revisions of "Part:BBa J364007"

(Usage and Biology)
(Usage and Biology)
Line 11: Line 11:
 
Fluorescence over Absorbance in LB media:  
 
Fluorescence over Absorbance in LB media:  
 
https://parts.igem.org/File:Abs_per_particle_with_(OD%3D0.02).jpg
 
https://parts.igem.org/File:Abs_per_particle_with_(OD%3D0.02).jpg
 +
and in TB media:
 +
  
  

Revision as of 14:43, 18 October 2019


Test Device 4 for 2018 InterLab Study

Test Device 4 expresses GFP under the control of a constitutive promoter from the Anderson collection.

Usage and Biology

E.coli GFP expression in LB and TB media: https://parts.igem.org/File:Figures_of_Device_4_and_Negative_control.xls Fluorescence over Absorbance in LB media: https://parts.igem.org/File:Abs_per_particle_with_(OD%3D0.02).jpg and in TB media:


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705