Difference between revisions of "Part:BBa K3228040"

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This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from iGEM Marburg 2019. Cyanobacteria show a different absorption and excitation behaviour than other bacterial cells due to their photosystems.Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set is a recommendation of reporters based on experimental data LLLLink to experiments with cyanosLLL and literature.
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This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from <a href="https://2019.igem.org/Team:Marburg">iGEM Marburg 2019.</a>Cyanobacteria show a different absorption and excitation behaviour than other bacterial cells due to their photosystems.Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set is a recommendation of reporters based on  
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<a href="https://2019.igem.org/Team:Marburg/Results">experimental data</a>and literature.
 
   
 
   
 
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These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs. For more information we recommend to head over to <Link to Design Marburg collection 2018>. <br>
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These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs. For more information we recommend to head over to <a href="http://2018.igem.org/Team:Marburg/Design"> Design of the Marburg Collection</a>.
Be aware that some of these reporters are codon optimized for Synechococcus elongatus UTEX2973.
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Revision as of 14:42, 18 October 2019


pMC_0_4_22_NanoLuc-K1159001

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 520
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

specific text here

This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from iGEM Marburg 2019.Cyanobacteria show a different absorption and excitation behaviour than other bacterial cells due to their photosystems.Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set is a recommendation of reporters based on experimental dataand literature.

Compability

These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection.