Difference between revisions of "Part:BBa K2915275:Design"

 
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===Design Notes===
 
===Design Notes===
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We add a 6Hig-Tag onto the "TAL2" sequence which allows us to purify our proteins.
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We add a 6Hig-Tag and a TeV cleavage site into the "TAL2" sequence which allows us to purify our proteins.
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We optimized the codons to be able to produce these proteins into the K12 E.coli strain. To perform the production f TAL2 in a K12 E.coli strain, we added a T7 promotor and a RBS into the psb1c3 plasmide.
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We optimized the codons to be able to produce these proteins into the K12 E.coli strain. To perform the production f TAL2 in a K12 E.coli strain, we added a T7 promotor and a RBS into the psb1c3 plasmide,which is the regulator..
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This biobrick is in RFC 10 standards with:
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The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC
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and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Cermak T, et al. Efficient design and assembly of custom TALEs and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res 2011 Sep 1;39(17):7879.

Latest revision as of 13:25, 18 October 2019


IPTG inducible promoter (T7) with RBS TAL2 6-His-Tag with TEV site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 246


Design Notes

We add a 6Hig-Tag and a TeV cleavage site into the "TAL2" sequence which allows us to purify our proteins. We optimized the codons to be able to produce these proteins into the K12 E.coli strain. To perform the production f TAL2 in a K12 E.coli strain, we added a T7 promotor and a RBS into the psb1c3 plasmide,which is the regulator.. This biobrick is in RFC 10 standards with: The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'

Source

Nucleotide sequence

References

Cermak T, et al. Efficient design and assembly of custom TALEs and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res 2011 Sep 1;39(17):7879.