This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from iGEM Marburg 2019. Cyanobacteria show a different absorption and excitation behaviour than other bacterial cells due to their photosystems.Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set is a recommendation of reporters based on experimental data LLLLink to experiments with cyanosLLL and literature.
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===Compability===
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These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs. For more information we recommend to head over to <Link to Design Marburg collection 2018>. <br>
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Be aware that some of these reporters are codon optimized for Synechococcus elongatus UTEX2973.
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===Functional Parameters===
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<partinfo>BBa_K3228040 parameters</partinfo>
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Revision as of 13:25, 18 October 2019
pMC_0_4_22_NanoLuc-K1159001
Sequence and Features
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 19
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4 Illegal AgeI site found at 520
1000
COMPATIBLE WITH RFC[1000]
Description
specific text here
This part is contained in a collection of reporters for Synechococcus elongatus UTEX2973 from iGEM Marburg 2019. Cyanobacteria show a different absorption and excitation behaviour than other bacterial cells due to their photosystems.Hence not every fluorescence based reporter can be recommended for the use in cyanobacteria. This set is a recommendation of reporters based on experimental data LLLLink to experiments with cyanosLLL and literature.
Compability
These parts are compatible with the RCF [1000] standard. These parts are compatible with any Part Collection that uses the Phytobrick standard of overhangs. For more information we recommend to head over to .
Be aware that some of these reporters are codon optimized for Synechococcus elongatus UTEX2973.