Difference between revisions of "Part:BBa K2915225:Design"

(→‎Design Notes)
(→‎References)
 
Line 14: Line 14:
  
 
===References===
 
===References===
 +
 +
Cermak T, et al. Efficient design and assembly of custom TALEs and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res 2011 Sep 1;39(17):7879.

Latest revision as of 13:25, 18 October 2019


IPTG inducible promoter (T7) with RBS TAL1 6-His-Tag with TEV site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We add a 6Hig-Tag and a TeV cleavage site into the "TAL1" sequence which allows us to purify our proteins. We optimized the codons to be able to produce these proteins into the K12 E.coli strain. To perform the production of TAL1 in a K12 E.coli strain, we added a T7 promotor and a RBS into the psb1c3 plasmide,which is the regulator.. This biobrick is in RFC 10 standards with: The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'

Source

Nucleotide sequence

References

Cermak T, et al. Efficient design and assembly of custom TALEs and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res 2011 Sep 1;39(17):7879.