Difference between revisions of "Part:BBa K2915275"

 
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<partinfo>BBa_K2915275 short</partinfo>
 
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The Talen 2 it's a Talen which came from a plant pathogenic bacteria in the genus Xanthomonas. These designed proteins can able to bind specific DNA fragment ( 5'CCGCTG3') such as the human amylase gene . This TAL2 can be purify because of the presence of a 6His-Tag in the N-terminal of the protein.
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The TAL2 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas. These designed proteins are able to bind itself specifically onto a DNA fragment ( 5'CCGCTG3') of a Tubercolusis gene . The promoter T7 and RBS (BBa_K525998) are regulator that are assembled with TAL1, which allows the production TAL1 with its HisTag using E.coli DH5-alpha bacteria. We also tested production of the protein and used the His-Tag to purify the protein and we tested the activity of our protein using a gel shift.
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<partinfo>BBa_K2915275 parameters</partinfo>
 
<partinfo>BBa_K2915275 parameters</partinfo>
 
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'''Design Notes'''
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This biobrick is in RFC 10 standards with:
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The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'
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This TAL2 exists with:
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- with a His-tag and a TEV site
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- with a promotor T7 and RBS (BBa_K525998) which is the regulator.

Revision as of 12:36, 18 October 2019


IPTG inducible promoter (T7) with RBS TAL2 6-His-Tag with TEV site

The TAL2 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas. These designed proteins are able to bind itself specifically onto a DNA fragment ( 5'CCGCTG3') of a Tubercolusis gene . The promoter T7 and RBS (BBa_K525998) are regulator that are assembled with TAL1, which allows the production TAL1 with its HisTag using E.coli DH5-alpha bacteria. We also tested production of the protein and used the His-Tag to purify the protein and we tested the activity of our protein using a gel shift.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 246


Design Notes

This biobrick is in RFC 10 standards with:

The prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC  and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3'

This TAL2 exists with:

- with a His-tag and a TEV site

- with a promotor T7 and RBS (BBa_K525998) which is the regulator.