Difference between revisions of "Part:BBa K2915225"
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<partinfo>BBa_K2915225 parameters</partinfo> | <partinfo>BBa_K2915225 parameters</partinfo> | ||
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+ | |||
+ | '''Design Notes''' | ||
+ | This biobrick is in RFC 10 standards with: | ||
+ | the prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC | ||
+ | and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3' | ||
+ | This TAL1 exists with: | ||
+ | - with a His-tag and a TEV site | ||
+ | - with a promotor T7 and RBS (BBa_K525998) which is the regulator. |
Revision as of 12:21, 18 October 2019
IPTG inducible promoter (T7) with RBS TAL1 6-His-Tag with TEV site
The TAL1 is a TALEN which came from a plant pathogenic bacteria in the genus Xanthomonas.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes This biobrick is in RFC 10 standards with: the prefixe (with ATG in frame): 5' GAATTC GCGGCCGC T TCTAGA TG GCCGGC and the suffixe (contain Stop in frame): ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG 3' This TAL1 exists with: - with a His-tag and a TEV site - with a promotor T7 and RBS (BBa_K525998) which is the regulator.