Difference between revisions of "Part:BBa K2921550"
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This construct constitutively expresses a colored metal-binding fusion protein: metallothionein (Basic part: BBa_K1460002) linked with mRFP (Basic part: BBa_E1010). Metallothionein is a metal-binding protein that can tightly chelate heavy metal ions by forming a strong coordination bond. The mRFP serves as a functional color reporter, allowing for the convenient visible or UV detection of the location of metallothionein. | This construct constitutively expresses a colored metal-binding fusion protein: metallothionein (Basic part: BBa_K1460002) linked with mRFP (Basic part: BBa_E1010). Metallothionein is a metal-binding protein that can tightly chelate heavy metal ions by forming a strong coordination bond. The mRFP serves as a functional color reporter, allowing for the convenient visible or UV detection of the location of metallothionein. | ||
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Revision as of 10:37, 18 October 2019
Metallothionein + EAAAK-linker + mRFP
This construct constitutively expresses a colored metal-binding fusion protein: metallothionein (Basic part: BBa_K1460002) linked with mRFP (Basic part: BBa_E1010). Metallothionein is a metal-binding protein that can tightly chelate heavy metal ions by forming a strong coordination bond. The mRFP serves as a functional color reporter, allowing for the convenient visible or UV detection of the location of metallothionein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 706
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1531
Illegal AgeI site found at 1643 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 118