Difference between revisions of "Part:BBa K1678007"
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This part can be used as a Vitamin B12 biosensor, we tested it for the AdoCobalamin form of B12 (AKA Coenzyme B12), but it might be used also to detect CyanoCobalamin, HydroxyCobalamin, MethylCobalamin according to the following publication : "A riboswitch sensor to determine vitamin B12 in fermented foods" | This part can be used as a Vitamin B12 biosensor, we tested it for the AdoCobalamin form of B12 (AKA Coenzyme B12), but it might be used also to detect CyanoCobalamin, HydroxyCobalamin, MethylCobalamin according to the following publication : "A riboswitch sensor to determine vitamin B12 in fermented foods" | ||
Xuan Zhu 2015. We didn't tried this because we were out of time, anyway this would have to be characterized. | Xuan Zhu 2015. We didn't tried this because we were out of time, anyway this would have to be characterized. | ||
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| + | =2019 OUC-China's characterization= | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:25, 18 October 2019
Vitamin B12 biosensor, composed by a cobalamin riboswitch in front of an mRFP1
This part can be used as a Vitamin B12 biosensor, we tested it for the AdoCobalamin form of B12 (AKA Coenzyme B12), but it might be used also to detect CyanoCobalamin, HydroxyCobalamin, MethylCobalamin according to the following publication : "A riboswitch sensor to determine vitamin B12 in fermented foods"
Xuan Zhu 2015. We didn't tried this because we were out of time, anyway this would have to be characterized.
2019 OUC-China's characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 964
Illegal AgeI site found at 1076 - 1000COMPATIBLE WITH RFC[1000]