Difference between revisions of "Part:BBa K2978101:Design"

 
 
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===Source===
 
===Source===
  
−
Synthesize <i>de novo</i> with codon optimization for <i>L. casei</i>
+
Synthesize <i>de novo</i> with codon optimization for <i>E. coli</i>
  
 
===References===
 
===References===
 +
Martin, M. J., Clare, S., Goulding, D., Faulds-Pain, A., Barquist, L., Browne, H. P., … Wren, B. W. (2013). The agr Locus Regulates Virulence and Colonization Genes in <i>Clostridium difficile</i> 027. <i>Journal of Bacteriology</i>, 195(16), 3672–3681. doi:10.1128/jb.00473-13.
 +
<br>
 +
 +
Lubkowicz, D., Ho, C. L., Hwang, I. Y., Yew, W. S., Lee, Y. S., & Chang, M. W. (2018). Reprogramming Probiotic <i>Lactobacillus reuteri</i> as a Biosensor for <i>Staphylococcus aureus</i> Derived AIP-I Detection. <i>ACS Synthetic Biology</i>, 7(5), 1229–1237. doi:10.1021/acssynbio.8b00063.
 +
<br>
 +
 +
Reynolds, J., & Wigneshweraraj, S. (2011). Molecular Insights into the Control of Transcription Initiation at the <i>Staphylococcus aureus</i> agr operon. <i>Journal of Molecular Biology</i>, 412(5), 862–881. doi:10.1016/j.jmb.2011.06.018.
 +
<br>
 +
 +
Rajasree, K., Fasim, A., & Gopal, B. (2016). Conformational features of the Staphylococcus aureus AgrA-promoter interactions rationalize quorum-sensing triggered gene expression. <i>Biochemistry and Biophysics Reports</i>, 6, 124–134. doi:10.1016/j.bbrep.2016.03.012.

Latest revision as of 06:38, 18 October 2019


AgrB Clostridioides difficile gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 370
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Synthesize de novo with codon optimization for E. coli

References

Martin, M. J., Clare, S., Goulding, D., Faulds-Pain, A., Barquist, L., Browne, H. P., … Wren, B. W. (2013). The agr Locus Regulates Virulence and Colonization Genes in Clostridium difficile 027. Journal of Bacteriology, 195(16), 3672–3681. doi:10.1128/jb.00473-13.

Lubkowicz, D., Ho, C. L., Hwang, I. Y., Yew, W. S., Lee, Y. S., & Chang, M. W. (2018). Reprogramming Probiotic Lactobacillus reuteri as a Biosensor for Staphylococcus aureus Derived AIP-I Detection. ACS Synthetic Biology, 7(5), 1229–1237. doi:10.1021/acssynbio.8b00063.

Reynolds, J., & Wigneshweraraj, S. (2011). Molecular Insights into the Control of Transcription Initiation at the Staphylococcus aureus agr operon. Journal of Molecular Biology, 412(5), 862–881. doi:10.1016/j.jmb.2011.06.018.

Rajasree, K., Fasim, A., & Gopal, B. (2016). Conformational features of the Staphylococcus aureus AgrA-promoter interactions rationalize quorum-sensing triggered gene expression. Biochemistry and Biophysics Reports, 6, 124–134. doi:10.1016/j.bbrep.2016.03.012.