Difference between revisions of "Part:BBa K2908675:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The construction method is the same as module1, only except the paired sequence of each inserted fragment, which shows in the following picture. | |
https://static.igem.org/mediawiki/parts/7/78/T--CSU_CHINA--ggmodule2.png | https://static.igem.org/mediawiki/parts/7/78/T--CSU_CHINA--ggmodule2.png | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
+ | Waclaw Szybalski”, Sun C. Kim”, Noaman Hasan” and Anna J. Podhajskab, Class IIS restriction enzymes, a review.1991, Gene, 100: 13-26. |
Latest revision as of 21:52, 17 October 2019
s(ESR1)p-miR101spe-EGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The construction method is the same as module1, only except the paired sequence of each inserted fragment, which shows in the following picture.
Source
We designed it by ourselves and constructed this part by Golden gate.
References
Waclaw Szybalski”, Sun C. Kim”, Noaman Hasan” and Anna J. Podhajskab, Class IIS restriction enzymes, a review.1991, Gene, 100: 13-26.