Difference between revisions of "Part:BBa K2908671"
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===Usage and Biology===
 
===Usage and Biology===
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- We use the pLN431[2] as the back-clone of the Module1, then we cut the vector and an insert sequence (lacZp+lacZ) with MluI and NotI to form the pocket plasmid.<br/>
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https://static.igem.org/mediawiki/parts/d/d2/T--CSU_CHINA--lacZ.png<br/>
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Revision as of 21:35, 17 October 2019


pLN431-s(GATA3)p-GAD-miR101-BS

The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of GAD and miR101-BS for sequence-specific expression of GAD.Therefore,we used our p(GATA3)p(BBa_K2908000) as a specific promoter. At the same time we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation domain of GAD.In this way we can ensure the specificity and independence of this protein. T--CSU_CHINA--module1.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 99
    Illegal NheI site found at 584
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 279