Difference between revisions of "Part:BBa K2908670"

 
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The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of yCD and miR101-BS for sequence-specific expression of yCD .Therefore, we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101 so that regulate the transactivation domain of yCD(BBa_K2908667).CD converts the prodrug 5-FC (used to treat infections by fungi such as Candida albicans and Cryptococcus neoformans) into the active agent 5-fluorouracil (5-FU). While 5-FC is nontoxic to human cells because of the lack of CD, 5-FU is used to treat cancers like colon, pancreatic, and breast cancer. The cytotoxic effects of 5-FU occur following its conversion to 5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP). 5-FdUMP is an irreversible inhibitor of thymidylate synthase and thus inhibits DNA synthesis by deoxythymidine triphosphate (dTTP) deprivation and causes DNA strand breakage, leading to cell death.
 
The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of yCD and miR101-BS for sequence-specific expression of yCD .Therefore, we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101 so that regulate the transactivation domain of yCD(BBa_K2908667).CD converts the prodrug 5-FC (used to treat infections by fungi such as Candida albicans and Cryptococcus neoformans) into the active agent 5-fluorouracil (5-FU). While 5-FC is nontoxic to human cells because of the lack of CD, 5-FU is used to treat cancers like colon, pancreatic, and breast cancer. The cytotoxic effects of 5-FU occur following its conversion to 5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP). 5-FdUMP is an irreversible inhibitor of thymidylate synthase and thus inhibits DNA synthesis by deoxythymidine triphosphate (dTTP) deprivation and causes DNA strand breakage, leading to cell death.
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https://static.igem.org/mediawiki/parts/1/18/T--CSU_CHINA--module3.png
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 21:27, 17 October 2019


pLN431-G8p-yCD-miR101BS

The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of yCD and miR101-BS for sequence-specific expression of yCD .Therefore, we used our miR101-BD(BBa_K2908668) to bind different concentrations of miR101 so that regulate the transactivation domain of yCD(BBa_K2908667).CD converts the prodrug 5-FC (used to treat infections by fungi such as Candida albicans and Cryptococcus neoformans) into the active agent 5-fluorouracil (5-FU). While 5-FC is nontoxic to human cells because of the lack of CD, 5-FU is used to treat cancers like colon, pancreatic, and breast cancer. The cytotoxic effects of 5-FU occur following its conversion to 5-fluoro-2'-deoxyuridine-5'-monophosphate (5-FdUMP). 5-FdUMP is an irreversible inhibitor of thymidylate synthase and thus inhibits DNA synthesis by deoxythymidine triphosphate (dTTP) deprivation and causes DNA strand breakage, leading to cell death. T--CSU_CHINA--module3.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 197
    Illegal NheI site found at 1143
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 244
    Illegal XhoI site found at 60
    Illegal XhoI site found at 174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 755
    Illegal BsaI.rc site found at 792