Difference between revisions of "Part:BBa K2908667:Design"
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===Design Notes=== | ===Design Notes=== | ||
We must use the sequence of it correctly or yCD cannot work effectively. | We must use the sequence of it correctly or yCD cannot work effectively. | ||
| − | + | https://static.igem.org/mediawiki/parts/f/f2/T--CSU_CHINA--yCD1.png | |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Ko, T.-P., Lin, J.-J., Hu, C.-Y., Hsu, Y.-H., Wang, A.H.-J., Liaw, S.-H, Crystal structure of yeast cytosine deaminase. Insights into enzyme mechanism and evolution, (2003) J.Biol.Chem. 278: 19111-19117. | ||
Latest revision as of 21:12, 17 October 2019
yCD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 854
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 466
Illegal BsaI.rc site found at 503
Design Notes
We must use the sequence of it correctly or yCD cannot work effectively.
Source
We get the sequence from NCBI and synthesized it by company.
References
Ko, T.-P., Lin, J.-J., Hu, C.-Y., Hsu, Y.-H., Wang, A.H.-J., Liaw, S.-H, Crystal structure of yeast cytosine deaminase. Insights into enzyme mechanism and evolution, (2003) J.Biol.Chem. 278: 19111-19117.