Difference between revisions of "Part:BBa K3013000"

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</html><partinfo>BBa_K3013000 short</partinfo><html>
 
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LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI.
 
LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI.
  
 
We used this part to tag polypropylene and polystyrene with eGFP.  
 
We used this part to tag polypropylene and polystyrene with eGFP.  
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Revision as of 17:06, 17 October 2019

</html>LCI, polymer binding peptide LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI. We used this part to tag polypropylene and polystyrene with eGFP. ===Usage and Biology=== Therefore we made a fusion protein: eGFP - 17x helix - LCI. This Construct was expressed under a T7 promoter in E. coli BL21. The soluble protein fraction was purified and binding was tested in microtiter plates. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
===Functional Parameters===
biology-NA-
function-NA-
protein-NA-

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