Difference between revisions of "Part:BBa K3282006"

Latest revision as of 16:49, 17 October 2019

Tac Promoter

Tac is a hybrid promoter, which has the consensus -35 sequence from the trp promoter and the consensus -10 sequence from the lac UV5 promoter. It is reported to be 5-10 times stronger than the lac UV5 promoter and is induced using IPTG and other galactosides while not being subject to catabolite repression (1). The higher efficiency of this hybrid promoter with respect to either one of the parental promoters is attributed to the presence of consensus Pribnow box sequence in addition to consensus -35 sequence (2). This part was used in composite parts BBa_K3282004 and BBa_K3282007

References

1. Donovan, R.S., Robinson, C.W. & Glick, B.R. Journal of Industrial Microbiology (1996) 16: 145.

2. de Boer, H. A., Comstock, L. J., & Vasser, M. (1983). The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences of the United States of America, 80(1), 21–25.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3282006 short</partinfo>
-Tac is a hybrid promoter, which has the consensus -35 sequence from the trp promoter and the consensus -10 sequence from the lac UV5 promoter. It is reported to be 5-10 times stronger than the lac UV5 promoter and is induced using IPTG and other galactosides while not being subject to catabolite repression (1). The higher efficiency of this hybrid promoter with respect to either one of the parental promoters is attributed to the presence of consensus Pribnow box sequence in addition to consensus -35 sequence (2).
+Tac is a hybrid promoter, which has the consensus -35 sequence from the trp promoter and the consensus -10 sequence from the lac UV5 promoter. It is reported to be 5-10 times stronger than the lac UV5 promoter and is induced using IPTG and other galactosides while not being subject to catabolite repression (1). The higher efficiency of this hybrid promoter with respect to either one of the parental promoters is attributed to the presence of consensus Pribnow box sequence in addition to consensus -35 sequence (2). This part was used in composite parts [https://parts.igem.org/Part:BBa_K3282004 BBa_K3282004] and [https://parts.igem.org/Part:BBa_K3282007 BBa_K3282007]
+References
+. Donovan, R.S., Robinson, C.W. & Glick, B.R. Journal of Industrial Microbiology (1996) 16: 145.
+. de Boer, H. A., Comstock, L. J., & Vasser, M. (1983). The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences of the United States of America, 80(1), 21–25.
 <!-- Add more about the biology of this part here