Difference between revisions of "Part:BBa K2983003"

 
 
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This part is the Ura3 gene of ''Yarrowia lipolytica''.
 
This part is the Ura3 gene of ''Yarrowia lipolytica''.
  
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===Usage and Biology===
 
===Usage and Biology===
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The Ura3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotides synthesis pathway. In the absence of this enzyme, the cells are able to grow only if uracil or uridine are present in the media.
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It is a wildly used auxotrophic selection marker in yeast.
  
 
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Latest revision as of 14:22, 17 October 2019


Ura3 gene

This part is the Ura3 gene of Yarrowia lipolytica.

Usage and Biology

The Ura3 gene encodes the orotidine 5'-phosphate decarboxylase, an enzyme (EC. 4.1.1.23) that catalyzes the decarboxylation of orotidine monophosphate to uridine monophosphate in the pyrimidine ribonucleotides synthesis pathway. In the absence of this enzyme, the cells are able to grow only if uracil or uridine are present in the media.

It is a wildly used auxotrophic selection marker in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 17
    Illegal XhoI site found at 50
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 205
    Illegal AgeI site found at 308
  • 1000
    COMPATIBLE WITH RFC[1000]