Difference between revisions of "Part:BBa K2924034"

Revision as of 14:16, 17 October 2019

Pcpc560 + α-s1-casein + T1/T7 Terminator

Strong constitutive cyanobacterial promoter P_cpc560 expressing a-s1-casein with the T1/T7 double terminator.

The promoter was cloned into the pSHDY plasmid. The pSHDY plasmid is an RSF1010-based, low-copy self-replicating vector derived from pVZ321 and has a broad host range, which can ensure the conjugation from E. coli to cyanobacteria and other microorganisms¹. After testing the strength of the promoter with a fluorescent reporter mVenus, it was used to express a protein from cow’s milk, a-s1-casein in Synechocystis sp. PCC 6803.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1204
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <html><p align="justify">
 Strong constitutive cyanobacterial promoter <a href="https://parts.igem.org/part:BBa_K2924000">P<sub>cpc560</sub></a> expressing <a href="https://parts.igem.org/part:BBa_K2924026">a-s1-casein</a> with the <a href="https://parts.igem.org/part:BBa_B0015">T1/T7 double terminator</a>.
-===Usage and Biology===
 <p align="justify">
 The promoter was cloned into the pSHDY plasmid. The pSHDY plasmid is an RSF1010-based, low-copy self-replicating vector derived from pVZ321 and has a broad host range, which can ensure the conjugation from <i>E. coli</i> to cyanobacteria and other microorganisms<sup>1</sup>.
 After testing the strength of the promoter with a fluorescent reporter <a href="https://parts.igem.org/part:BBa_K2924036">mVenus</a>, it was used to express a protein from cow’s milk, <a href="https://parts.igem.org/part:BBa_K2924026">a-s1-casein</a> in <i>Synechocystis</i> sp. PCC 6803.
 </html>
+===Usage and Biology===