Difference between revisions of "Part:BBa K2984013"
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One restricting factor for cultivating microalgae is the high risk of contamination with bacteria, fungi or zooplankton. As our Team anticipates in building a bioreactor it is essential to sterilize the media and all containers which raises the costs of production. Loera-Quezada et al. (2016) found a way to bypass this problem by integrating <i>Pseudomonas stutzeri</i> WM88 gene ptxD in <i>C. reinhardtii</i>. It encodes a phosphite oxidoreductase which is able to oxidize phosphite into phosphate using NAD+ as a cofactor (White & Metcalf, 2007). The integration of ptxD into the genome of <i>C. reinhardtii</i> enables the use of phosphite as a sole phosphorus source which wasn’t possible before (Loera-Quezada et al., 2015). Loera-Quezada et al. (2016) presented a new way of microalgae production without the need for sterile conditions or antibiotics to avoid contamination with biological pollutants as they can’t use phosphite as a phosphorus source. In the future this system could also by applied to open pond systems.
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<partinfo>BBa_K2984013 short</partinfo>
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This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. One restricting factor for cultivating microalgae is the high risk of contamination with bacteria, fungi or zooplankton. As our Team anticipates in building a bioreactor it is essential to sterilize the media and all containers which raises the costs of production. Loera-Quezada et al. (2016) found a way to bypass this problem by integrating <i>Pseudomonas stutzeri</i> WM88 gene ptxD in <i>C. reinhardtii</i>. It encodes a phosphite oxidoreductase which is able to oxidize phosphite into phosphate using NAD+ as a cofactor (White & Metcalf, 2007). The integration of ptxD into the genome of <i>C. reinhardtii</i> enables the use of phosphite as a sole phosphorus source which wasn’t possible before (Loera-Quezada et al., 2015). Loera-Quezada et al. (2016) presented a new way of microalgae production without the need for sterile conditions or antibiotics to avoid contamination with biological pollutants as they can’t use phosphite as a phosphorus source. In the future this system could also by applied to open pond systems.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2984005 SequenceAndFeatures</partinfo>

Revision as of 14:06, 17 October 2019

Phosphite oxidoreductase (ptxD) B3-B4

This part is a part of the Chlamy-HUB-Collection. One restricting factor for cultivating microalgae is the high risk of contamination with bacteria, fungi or zooplankton. As our Team anticipates in building a bioreactor it is essential to sterilize the media and all containers which raises the costs of production. Loera-Quezada et al. (2016) found a way to bypass this problem by integrating Pseudomonas stutzeri WM88 gene ptxD in C. reinhardtii. It encodes a phosphite oxidoreductase which is able to oxidize phosphite into phosphate using NAD+ as a cofactor (White & Metcalf, 2007). The integration of ptxD into the genome of C. reinhardtii enables the use of phosphite as a sole phosphorus source which wasn’t possible before (Loera-Quezada et al., 2015). Loera-Quezada et al. (2016) presented a new way of microalgae production without the need for sterile conditions or antibiotics to avoid contamination with biological pollutants as they can’t use phosphite as a phosphorus source. In the future this system could also by applied to open pond systems.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 729
    Illegal XhoI site found at 721
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal AgeI site found at 1535
    Illegal AgeI site found at 1647
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1823
    Illegal BsaI.rc site found at 752