Difference between revisions of "Part:BBa K2984014"
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− | The His-Tag is an amino acid motif at the C- or N-terminus of proteins. It is one of the most commonly used purification tags with a minimum of six or more consecutive histidine residues. They are able to form complexes with metal ions like Ni2<sup>+</sup> or Co2<sup>+</sup> making it possible to purify the proteins attached to the his-tag when those transition metal ions are connected on beads. The amino acid sequence is HHHHHH. | + | <html> |
+ | This part is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. The His-Tag is an amino acid motif at the C- or N-terminus of proteins. It is one of the most commonly used purification tags with a minimum of six or more consecutive histidine residues. They are able to form complexes with metal ions like Ni2<sup>+</sup> or Co2<sup>+</sup> making it possible to purify the proteins attached to the his-tag when those transition metal ions are connected on beads. The amino acid sequence is HHHHHH. | ||
+ | </html> | ||
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Revision as of 14:02, 17 October 2019
6x His-Tag for protein detection, isolation, purification B5-B5
This part is a part of the Chlamy-HUB-Collection. The His-Tag is an amino acid motif at the C- or N-terminus of proteins. It is one of the most commonly used purification tags with a minimum of six or more consecutive histidine residues. They are able to form complexes with metal ions like Ni2+ or Co2+ making it possible to purify the proteins attached to the his-tag when those transition metal ions are connected on beads. The amino acid sequence is HHHHHH.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]