Difference between revisions of "Part:BBa K2924034"
| Line 5: | Line 5: | ||
Strong constitutive cyanobacterial promoter <a href="https://parts.igem.org/part:BBa_K2924000">P<sub>cpc560</sub></a> expressing <a href="https://parts.igem.org/part:BBa_K2924026">a-s1-casein</a> with the <a href="https://parts.igem.org/part:BBa_B0015">T1/T7 double terminator</a>. | Strong constitutive cyanobacterial promoter <a href="https://parts.igem.org/part:BBa_K2924000">P<sub>cpc560</sub></a> expressing <a href="https://parts.igem.org/part:BBa_K2924026">a-s1-casein</a> with the <a href="https://parts.igem.org/part:BBa_B0015">T1/T7 double terminator</a>. | ||
| − | + | <p align="justify"> | |
The promoter was cloned into the pSHDY plasmid. The pSHDY plasmid is an RSF1010-based, low-copy self-replicating vector derived from pVZ321 and has a broad host range, which can ensure the conjugation from <i>E. coli</i> to cyanobacteria and other microorganisms<sup>1</sup>. | The promoter was cloned into the pSHDY plasmid. The pSHDY plasmid is an RSF1010-based, low-copy self-replicating vector derived from pVZ321 and has a broad host range, which can ensure the conjugation from <i>E. coli</i> to cyanobacteria and other microorganisms<sup>1</sup>. | ||
After testing the strength of the promoter with a fluorescent reporter <a href="https://parts.igem.org/part:BBa_K2924036">mVenus</a>, it was used to express a protein from cow’s milk, <a href="https://parts.igem.org/part:BBa_K2924026">a-s1-casein</a> in <i>Synechocystis</i> sp. PCC 6803. | After testing the strength of the promoter with a fluorescent reporter <a href="https://parts.igem.org/part:BBa_K2924036">mVenus</a>, it was used to express a protein from cow’s milk, <a href="https://parts.igem.org/part:BBa_K2924026">a-s1-casein</a> in <i>Synechocystis</i> sp. PCC 6803. | ||
Revision as of 13:54, 17 October 2019
Pcpc560 + α-s1-casein + T1/T7 Terminator
Strong constitutive cyanobacterial promoter Pcpc560 expressing a-s1-casein with the T1/T7 double terminator.
The promoter was cloned into the pSHDY plasmid. The pSHDY plasmid is an RSF1010-based, low-copy self-replicating vector derived from pVZ321 and has a broad host range, which can ensure the conjugation from E. coli to cyanobacteria and other microorganisms1.
After testing the strength of the promoter with a fluorescent reporter mVenus, it was used to express a protein from cow’s milk, a-s1-casein in Synechocystis sp. PCC 6803.
===Usage and Biology===
Sequence and Features