Difference between revisions of "Part:BBa K2950000"

Revision as of 13:27, 17 October 2019

pYES-GAL1 promoter-OKS-TDH1 terminator-TEF1 promoter-ZhuI-CYC1 terminator

In our study, we aim to achieve carminic acid yield by S. cerevisiae, so we should firstly realize the production of the flavokermesic acid which is an intermediate between acetyl-CoA or malonyl-CoA and carminic acid in the biosynthesis pathway. <img src=""> </img> At the beginning, we transformed a plasmid with pYES as the copy vector to keep high copy number, the Aloe arborescens octaketide synthase (OKS) to synthesize non-reduced linear octaketide, and the ZhuI cyclase to convert non-reduced linear octaketide to flavokermesic acid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1769
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 150
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 In our study, we aim to achieve carminic acid yield by S. cerevisiae, so we should firstly realize the production of the flavokermesic acid which is an intermediate between acetyl-CoA or malonyl-CoA and carminic acid in the biosynthesis pathway.
+<img src="https://2019.igem.org/wiki/images/2/2f/T--BNDS_China--composite_about_OKS-ZhuI.png"> </img>
 At the beginning, we transformed a plasmid with pYES as the copy vector to keep high copy number, the Aloe arborescens octaketide synthase (OKS) to synthesize non-reduced linear octaketide, and the ZhuI cyclase to convert non-reduced linear octaketide to flavokermesic acid.