Difference between revisions of "Part:BBa K2984035"

 
 
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<partinfo>BBa_K2984035 short</partinfo>
 
<partinfo>BBa_K2984035 short</partinfo>
  
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L1c plasmid containing PETase Enzyme fused to  mVEnus fluorescent marker for fluorecence meausrements of PETase concentration and His-tag for purification. The parts are flanked by the Psad promoter and the Rbcs2 terminator.  
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The construct is desgined to measure the erxpression level of PETase.
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1c plasmid containing <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">PETase enzyme</a>, fused to  <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">mVenus</a> fluorescent marker for fluorecence meausrements of PETase concentration and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984014">His-tag</a> for purification. The parts are flanked by the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The part has a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a> connecting the PsaD promoter and the PEtase.  
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The construct is desgined to measure the expression level of PETase.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2984035 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984035 SequenceAndFeatures</partinfo>
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==Characterization==
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<figure>
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<img src="https://2019.igem.org/wiki/images/9/95/T--Humboldt_Berlin--Konstrukt_8.jpeg" width="500">
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<figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii</figcaption>
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</figure>
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Latest revision as of 13:05, 17 October 2019


L1c-Psad-PETase-YFP-His-RbcS2

This vector is a part of the Chlamy-HUB-Collection. L1c plasmid containing PETase enzyme, fused to mVenus fluorescent marker for fluorecence meausrements of PETase concentration and His-tag for purification. The parts are flanked by the PsaD promoter and the Rbcs2 terminator. The part has a B2-B2 linker connecting the PsaD promoter and the PEtase. The construct is desgined to measure the expression level of PETase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1705
    Illegal PstI site found at 1919
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1244
    Illegal PstI site found at 1705
    Illegal PstI site found at 1919
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1705
    Illegal PstI site found at 1919
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1705
    Illegal PstI site found at 1919
    Illegal NgoMIV site found at 2396
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii