Difference between revisions of "Part:BBa K2924034"
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<partinfo>BBa_K2924034 short</partinfo> | <partinfo>BBa_K2924034 short</partinfo> | ||
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| + | Strong constitutive cyanobacterial promoter [https://parts.igem.org/part:BBa_K2924000 Pcpc560] expressing [https://parts.igem.org/part:BBa_K2924026 a-s1-casein] with the [https://parts.igem.org/part:BBa_B0015 T1/T7 double terminator] | ||
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| − | + | The promoter was cloned into the pSHDY plasmid. The pSHDY plasmid is an RSF1010-based, low-copy self-replicating vector derived from pVZ321 and has a broad host range, which can ensure the conjugation from <i>E. coli</i> to cyanobacteria and other microorganisms<sup>1</sup>. | |
| − | + | After testing the strength of the promoter with a fluorescent reporter (BBa_K2924036), it was used to express a protein from cow’s milk, a-s1-casein (BBa_K2924026) in <i>Synechocystis</i> sp. PCC 6803. | |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:57, 17 October 2019
Pcpc560 + α-s1-casein + T1/T7 Terminator
Strong constitutive cyanobacterial promoter Pcpc560 expressing a-s1-casein with the T1/T7 double terminator
The promoter was cloned into the pSHDY plasmid. The pSHDY plasmid is an RSF1010-based, low-copy self-replicating vector derived from pVZ321 and has a broad host range, which can ensure the conjugation from E. coli to cyanobacteria and other microorganisms1. After testing the strength of the promoter with a fluorescent reporter (BBa_K2924036), it was used to express a protein from cow’s milk, a-s1-casein (BBa_K2924026) in Synechocystis sp. PCC 6803.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1204
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]