Difference between revisions of "Part:BBa K3187012"

Revision as of 12:10, 17 October 2019

pTet sfGFP-P22 Bacteriophage Scaffolding Protein x Coat Protein pT7 (Convergent)

Name: pTeTW3con2-ptet-sfGFP-StrepTag-SP—CP+LPETGG-pT7
Size [bp]: 2932
Size [kDa]: 46.1 (sfGFP – Strep-Tag – Scaffold Protein) + 48.9 (Coat Protein - Strep-Tag - LPETGG)
Origin: Synthetic?
Components: tetA promoter, T7 promoter, T7 terminator, Lac Operator, RBS, Coat Protein, Scaffold Protein, sfGFP, LPETGG, Strep-Tag II
Feature: Production of P22 Virus-like particles with a modifiable surface and sfGFP as cargo

z

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 76
Illegal XbaI site found at 2900
Illegal PstI site found at 1334
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 76
Illegal NheI site found at 1339
Illegal PstI site found at 1334
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 76
Illegal BamHI site found at 796
Illegal XhoI site found at 1499
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 76
Illegal XbaI site found at 2900
Illegal PstI site found at 1334
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 76
Illegal XbaI site found at 2900
Illegal PstI site found at 1334
Illegal NgoMIV site found at 1185
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 94

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