Difference between revisions of "Part:BBa K2984032"

Revision as of 09:32, 17 October 2019

L1c-PsaD-ARS-PETase-3xHA-RBCS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, the ARS secretion signal, the PETase enzyme, a 3xHA tag, and the Rbcs2 terminator. The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1769
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1308
Illegal PstI site found at 1769
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
Illegal XhoI site found at 1032
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1769
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1769
1000
COMPATIBLE WITH RFC[1000]

Characterization

colonies_total — Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii

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-This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> secretion signal, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">PETase enzyme</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984015">3xHA tag</a>, and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.
+This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> secretion signal, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">PETase enzyme</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984015">3xHA tag</a>, and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.
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