Difference between revisions of "Part:BBa K2984030"

Revision as of 09:20, 17 October 2019

L1c-Psad-ARS-YFP-SP20-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. L1 construct containg arylsulfatase secretion Signal ARS, mVenus fluorescent marker, SP20 glycomodule. These parts are flanked by the PsaD promoter and the Rbcs2 terminator. The construct can be used for secretion analysis in C.reinhardtii. The glycomodule is supposed to ramp up secretion and post translational glycosilations.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1110
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1110
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1110
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1110
Illegal NgoMIV site found at 1587
1000
COMPATIBLE WITH RFC[1000]

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-This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1 construc containg Arylsulfatase secretion Signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> fluorescent marker, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">SP20</a> glycomodule. These parts are flanked by the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The construct can be used for secretion analysis in C.reinhardtii. The glycomodule is supposed to ramp up secretion and post translational glycosilations.
+This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1 construct containg arylsulfatase secretion Signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> fluorescent marker, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">SP20</a> glycomodule. These parts are flanked by the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The construct can be used for secretion analysis in C.reinhardtii. The glycomodule is supposed to ramp up secretion and post translational glycosilations.
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