Difference between revisions of "Part:BBa K2984027"
Line 3: Line 3:
 
<partinfo>BBa_K2984027 short</partinfo>
 
<partinfo>BBa_K2984027 short</partinfo>
  
−
<html>
+
 
 
L1c plasmid containg arylsulfatase secretion signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">mVenus</a> fluorescent marker which are flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 Terminator</a>. The construct is designed to test for the efficency of the secretion signal and the locus dependent secretion via fluorescence intensity measurements.
 
L1c plasmid containg arylsulfatase secretion signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">mVenus</a> fluorescent marker which are flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 Terminator</a>. The construct is designed to test for the efficency of the secretion signal and the locus dependent secretion via fluorescence intensity measurements.
−
</html5>
 
  
−
=='''Characterization'''==
 
  
−
<html>
+
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K2984027 SequenceAndFeatures</partinfo>
 +
 
 +
==Characterization==
 +
 
 +
 
 
<figure>
 
<figure>
 
<img src="igem" alt="colonies_total" width="500">
 
<img src="igem" alt="colonies_total" width="500">
 
<figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct could then be isolated from E. coli to be transformed in C. reinhardtii</figcaption>
 
<figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct could then be isolated from E. coli to be transformed in C. reinhardtii</figcaption>
 
</figure>
 
</figure>
−
</html>
 
  
−
<!-- -->
+
 
−
<span class='h3bb'>Sequence and Features</span>
+
 
−
<partinfo>BBa_K2984027 SequenceAndFeatures</partinfo>
+
  
  

Revision as of 08:42, 17 October 2019


L1c-Psad-ARS-YFP-Rbcs2


L1c plasmid containg arylsulfatase secretion signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">mVenus</a> fluorescent marker which are flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 Terminator</a>. The construct is designed to test for the efficency of the secretion signal and the locus dependent secretion via fluorescence intensity measurements.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1110
    Illegal NgoMIV site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

<figure> <img src="igem" alt="colonies_total" width="500"> <figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct could then be isolated from E. coli to be transformed in C. reinhardtii</figcaption> </figure>