Difference between revisions of "Part:BBa J364000"
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| − | ==Team NAWI_Graz 2019: GFP dynamics of | + | ==Team NAWI_Graz 2019: GFP dynamics of transformed <i>Escherichia coli</i> strains in LB broth== |
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<i>Background</i><br><br> | <i>Background</i><br><br> | ||
In <a href="https://2019.igem.org/Team:NAWI_Graz" target="_blank">Team NAWI_Graz 2019</a> | In <a href="https://2019.igem.org/Team:NAWI_Graz" target="_blank">Team NAWI_Graz 2019</a> | ||
| − | characterization, we found in normal growth/incubation condition (37 <sup>o</sup>C, LB agar) | + | characterization, we found that in normal growth/incubation condition (37 <sup>o</sup>C, LB agar) |
| − | BBa_J364000-transformed <i>Escherichia coli</i> BL21 | + | BBa_J364000-transformed <i>Escherichia coli</i> BL21 cells had the highest GFP expression in comparison to BL21 Star and Top 10 strains. In order to confirm that we have measured the GFP Flourescence of those three strains over 44 hours period. <br><br> |
<i>Experimental Design</i><br><br> | <i>Experimental Design</i><br><br> | ||
We used three different strains of transformed <i>E. coli</i> (Top10,BL21 and BL21 Star) for this study. | We used three different strains of transformed <i>E. coli</i> (Top10,BL21 and BL21 Star) for this study. | ||
| − | Transformed | + | Transformed strains were incubated in 15 mL LB broth 37 <sup>o</sup>C overnight. Next day they were used to inoculate a 50 mL LB to OD<sub>600</sub>=0.1. The cultures were then incubated at 37 <sup>o</sup>C, 140 rpm and samples were taken every 4 hours for 2 days to determine the green fluorescence protein (GFP) fluorescence intensity at 510 nm. |
<br><br> | <br><br> | ||
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<li>There are significant differences of GFP expression in different strains of <i>E. coli</i> | <li>There are significant differences of GFP expression in different strains of <i>E. coli</i> | ||
(Top10, BL21, BL21 Star)</li> | (Top10, BL21, BL21 Star)</li> | ||
| − | <li>The broth | + | <li>The broth became ligh green in color under natural light after around 14-18 hours of incubation time.</li> |
</ul> | </ul> | ||
Revision as of 07:39, 17 October 2019
Test Device 1 for the iGEM InterLab Study
This is a GFP expressing constitutive device for the 2017 iGEM InterLab study. It is called Test Device 1 for the study for easy reference.
This device is stored in pSB1C3 for the InterLab and is fully BioBrick compatible.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
Team NAWI_Graz 2019: GFP dynamics of transformed Escherichia coli strains in LB broth
In Team NAWI_Graz 2019
characterization, we found that in normal growth/incubation condition (37 oC, LB agar)
BBa_J364000-transformed Escherichia coli BL21 cells had the highest GFP expression in comparison to BL21 Star and Top 10 strains. In order to confirm that we have measured the GFP Flourescence of those three strains over 44 hours period.
Experimental Design
We used three different strains of transformed E. coli (Top10,BL21 and BL21 Star) for this study.
Transformed strains were incubated in 15 mL LB broth 37 oC overnight. Next day they were used to inoculate a 50 mL LB to OD600=0.1. The cultures were then incubated at 37 oC, 140 rpm and samples were taken every 4 hours for 2 days to determine the green fluorescence protein (GFP) fluorescence intensity at 510 nm.
Result and Findings
- There are significant differences of GFP expression in different strains of E. coli (Top10, BL21, BL21 Star)
- The broth became ligh green in color under natural light after around 14-18 hours of incubation time.
