Difference between revisions of "Part:BBa K3114008"

Revision as of 04:10, 17 October 2019

Chlorophyll B Reductase (CBR)

Chlorophyll B Reductase is a naturally occurring plant enzyme, encoded by the gene NYC1, which catalyzes the first step of chlorophyll b degradation (Horie et al. 2009). With the cofactor NADPH, chlorophyll b reductase converts chlorophyll b into 7-Hydroxymethyl Chlorophyll a.

Usage and Biology

BBa_K3114008 is a golden gate compatible part which allows insertion of the NYC1 gene into genetic circuits to express the enzyme chlorophyll B reductase.

Collection of Parts

This part was designed to be Golden Gate compatible with certain Golden Gate parts in iGEM Calgary's 2019 part collection including the following:
1. DsbA signal peptide - Bba_K3114000 https://parts.igem.org/Part:BBa_K3114000
2. MalE signal peptide - Bba_K3114001 https://parts.igem.org/Part:BBa_K3114001
3. OmpA signal peptide - Bba_K3114002 https://parts.igem.org/Part:BBa_K3114002
4. PhoA signal peptide - Bba_K3114003 https://parts.igem.org/Part:BBa_K3114003
5. Tat signal peptide - Bba_K3114004 https://parts.igem.org/Part:BBa_K3114004
6. TorA signal peptide - Bba_K3114005 https://parts.igem.org/Part:BBa_K3114005
7. Double Terminator - Bba_K3114013 https://parts.igem.org/Part:BBa_K3114013

References

Horie, Y., Ito, H., Kusaba, M., Tanaka, R., & Tanaka, A. (2009). Participation of chlorophyll b reductase in the initial step of the degradation of light-harvesting chlorophyll a/b-protein complexes in Arabidopsis. Journal of Biological Chemistry, 284(26), 17449-17456.

Sequence and Features