Difference between revisions of "Part:BBa K2957099"
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| + | Contents: hEF1a, IL-8 (CXCL-8), SynthpA, with Carb resistance | ||
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What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression | What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression | ||
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What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. | What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. | ||
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How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. " | How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. " | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| − | <partinfo> | + | <partinfo>BBa_K2957001 SequenceAndFeatures</partinfo> |
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===Functional Parameters=== | ===Functional Parameters=== | ||
| − | <partinfo> | + | <partinfo>BBa_K2957001 parameters</partinfo> |
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Latest revision as of 03:41, 17 October 2019
IL-8 (CXCL-8) with FLAG Tag
Contents: hEF1a, IL-8 (CXCL-8), SynthpA, with Carb resistance
What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression
What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.
How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. "
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 244
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]