Difference between revisions of "Part:BBa K2010999"
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ATGAATTTTCCTCGCGCTTCACGACTGATGCAGGCTGCCGTTCTTGGAGGGCTGATGGCCGTTAGCGCGGCCGCCACCGCTCAGACAAATCCCTACGCCCGCGGTCCGAATCCGACAGCCGCCAGTTTGGAAGCGAGCGCTGGTCCATTCACCGTTCGCTCCTTTACCGTGAGTAGACCGAGCGGTTATGGCGCTGGCACCGTTTACTATCCAACAAATGCTGGGGGTACCGTGGGCGCCATAGCCATAGTTCCCGGGTATACGGCACGGCAGTCATCAATTAAATGGTGGGGACCGCGTCTGGCATCCCACGGTTTCGTAGTAATTACAATTGACACAAATTCCACGTTAGACCAGCCATCAAGTCGGAGTTCGCAACAAATGGCCGCGCTGCGCCAGGTGGCGTCGTTAAACGGTACAAGTAGCAGCCCGATTTACGGAAAGGTCGATACCGCTCGTATGGGTGTTATGGGGTGGAGTATGGGAGGTGGAGGCTCCCTGATCTCTGCTGCTAACAACCCTTCGCTGAAAGCAGCGGCGCCTCAAGCACCATGGGATTCTTCGACAAATTTTAGTTCTGTAACTGTGCCCACGCTGATCTTCGCATGTGAAAACGATAGTATAGCCCCGGTCAACTCTTCAGCACTTCCTATCTATGATTCTATGTCACGCAACGCTAAGCAGTTTCTCGAAATTAATGGTGGCTCACATTCCTGTGCGAATAGCGGCAATTCTAACCAAGCATTAATCGGAAAAAAAGGCGTTGCATGGATGAAACGTTTTATGGACAATGATACTAGGTATTCTACTTTTGCCTGCGAGAACCCGAATAGCACCAGAGTGTCTGATTTTCGTACAGCGAATTGCAGCTGA | ATGAATTTTCCTCGCGCTTCACGACTGATGCAGGCTGCCGTTCTTGGAGGGCTGATGGCCGTTAGCGCGGCCGCCACCGCTCAGACAAATCCCTACGCCCGCGGTCCGAATCCGACAGCCGCCAGTTTGGAAGCGAGCGCTGGTCCATTCACCGTTCGCTCCTTTACCGTGAGTAGACCGAGCGGTTATGGCGCTGGCACCGTTTACTATCCAACAAATGCTGGGGGTACCGTGGGCGCCATAGCCATAGTTCCCGGGTATACGGCACGGCAGTCATCAATTAAATGGTGGGGACCGCGTCTGGCATCCCACGGTTTCGTAGTAATTACAATTGACACAAATTCCACGTTAGACCAGCCATCAAGTCGGAGTTCGCAACAAATGGCCGCGCTGCGCCAGGTGGCGTCGTTAAACGGTACAAGTAGCAGCCCGATTTACGGAAAGGTCGATACCGCTCGTATGGGTGTTATGGGGTGGAGTATGGGAGGTGGAGGCTCCCTGATCTCTGCTGCTAACAACCCTTCGCTGAAAGCAGCGGCGCCTCAAGCACCATGGGATTCTTCGACAAATTTTAGTTCTGTAACTGTGCCCACGCTGATCTTCGCATGTGAAAACGATAGTATAGCCCCGGTCAACTCTTCAGCACTTCCTATCTATGATTCTATGTCACGCAACGCTAAGCAGTTTCTCGAAATTAATGGTGGCTCACATTCCTGTGCGAATAGCGGCAATTCTAACCAAGCATTAATCGGAAAAAAAGGCGTTGCATGGATGAAACGTTTTATGGACAATGATACTAGGTATTCTACTTTTGCCTGCGAGAACCCGAATAGCACCAGAGTGTCTGATTTTCGTACAGCGAATTGCAGCTGA | ||
| − | + | ^ Updated 20191016 by Victoria Sajtovich of iGEM Toronto 2019 (due to the sequence's absence) | |
| + | iGEM HQ: see correspondence to William Cho and traci | ||
Revision as of 03:01, 17 October 2019
PETase (PET-degrading enzyme, origin I. sakaiensis)
PETase is the PET-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.
ATGAATTTTCCTCGCGCTTCACGACTGATGCAGGCTGCCGTTCTTGGAGGGCTGATGGCCGTTAGCGCGGCCGCCACCGCTCAGACAAATCCCTACGCCCGCGGTCCGAATCCGACAGCCGCCAGTTTGGAAGCGAGCGCTGGTCCATTCACCGTTCGCTCCTTTACCGTGAGTAGACCGAGCGGTTATGGCGCTGGCACCGTTTACTATCCAACAAATGCTGGGGGTACCGTGGGCGCCATAGCCATAGTTCCCGGGTATACGGCACGGCAGTCATCAATTAAATGGTGGGGACCGCGTCTGGCATCCCACGGTTTCGTAGTAATTACAATTGACACAAATTCCACGTTAGACCAGCCATCAAGTCGGAGTTCGCAACAAATGGCCGCGCTGCGCCAGGTGGCGTCGTTAAACGGTACAAGTAGCAGCCCGATTTACGGAAAGGTCGATACCGCTCGTATGGGTGTTATGGGGTGGAGTATGGGAGGTGGAGGCTCCCTGATCTCTGCTGCTAACAACCCTTCGCTGAAAGCAGCGGCGCCTCAAGCACCATGGGATTCTTCGACAAATTTTAGTTCTGTAACTGTGCCCACGCTGATCTTCGCATGTGAAAACGATAGTATAGCCCCGGTCAACTCTTCAGCACTTCCTATCTATGATTCTATGTCACGCAACGCTAAGCAGTTTCTCGAAATTAATGGTGGCTCACATTCCTGTGCGAATAGCGGCAATTCTAACCAAGCATTAATCGGAAAAAAAGGCGTTGCATGGATGAAACGTTTTATGGACAATGATACTAGGTATTCTACTTTTGCCTGCGAGAACCCGAATAGCACCAGAGTGTCTGATTTTCGTACAGCGAATTGCAGCTGA ^ Updated 20191016 by Victoria Sajtovich of iGEM Toronto 2019 (due to the sequence's absence) iGEM HQ: see correspondence to William Cho and traci
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 67
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]