Difference between revisions of "Part:BBa K3292001"

Revision as of 02:17, 17 October 2019

T7-LacO Promoter + strong RBS + sialidase + sfGFP + 6x his-tag + double terminator

This device uses Bba_K2406020, Bba_J34801 and Bba_0010 to regulate the expression of a super folding GFP (Bba_I746916). The main objective of creating this device is to facilitate the secretion and characterization of any protein intracellularly produced after being adding its nucleotide next to the sfGFP. In our case, we decided to add the coding sequence of sialidase from M. viridifaciens. The CDS of this enzymes comes from Micromonospora viridifaciens and was optimized for its expression in E. coli BL21 cells. Encoded by the gen ned A, this enzyme releases sialic acids (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids for use as carbon and energy sources due to its hydrolytic activity.

Additionally, for the proper characterization of this part, the sequence of the of the sfGFP was added to the construct. e of the of the sfGFP was added to the construct.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 721
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1986
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 646
Illegal BsaI.rc site found at 510
Illegal BsaI.rc site found at 1647
Illegal SapI.rc site found at 2055

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3292001 short</partinfo>
-This device uses Bba_K2406020, Bba_J34801 and Bba_0010 to regulate the expression of a super folding GFP (Bba_I746916). The main objective of creating this device is to facilitate the characterization of any coding sequence added next to the sfGFP. In our case, we decided to add the coding sequence of sialidase from M. viridifaciens.
+This device uses Bba_K2406020, Bba_J34801 and Bba_0010 to regulate the expression of a super folding GFP (Bba_I746916). The main objective of creating this device is to facilitate the secretion and characterization of any protein intracellularly produced after being adding its nucleotide next to the sfGFP. In our case, we decided to add the coding sequence of sialidase from M. viridifaciens.
 The CDS  of this enzymes comes from Micromonospora viridifaciens and was optimized for its expression in E. coli BL21 cells. Encoded by the gen ned A, this enzyme releases sialic acids (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids for use as carbon and energy sources due to its hydrolytic activity.
 Additionally, for the proper characterization of this part, the sequence of the of the  sfGFP was added to the construct.
+e of the of the  sfGFP was added to the construct.
 <!-- Add more about the biology of this part here