Difference between revisions of "Part:BBa E0022"

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<ECUST_China 2019 Charaterization>
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===ECUST_China 2019 Charaterization===
 
In ECUST_China 2019 characterization,we cloned pLAC to pet-28b and added eCFP as the reporter.
 
In ECUST_China 2019 characterization,we cloned pLAC to pet-28b and added eCFP as the reporter.

Revision as of 02:14, 17 October 2019

enhanced cyan fluorescent protein derived from A. victoria GFP

Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


ECUST_China 2019 Charaterization

In ECUST_China 2019 characterization,we cloned pLAC to pet-28b and added eCFP as the reporter.