Difference between revisions of "Part:BBa K3292001"
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<partinfo>BBa_K3292001 short</partinfo>
 
<partinfo>BBa_K3292001 short</partinfo>
  
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This device uses Bba_K2406020, Bba_K2406020 and Bba_B0010 to regulate the expression of a sialidase from M. viridifaciens. The coding sequence for this enzyme comes from Micromonospora viridifaciens and was optimized for its expression in E. coli BL21 cells.  
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This device uses Bba_K2406020, Bba_J34801 and Bba_0010 to regulate the expression of a super folding GFP (Bba_I746916). The main objective of creating this device is to facilitate the characterization of any coding sequence added next to the sfGFP. In our case, we decided to add the coding sequence of sialidase from M. viridifaciens.
  
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Encoded by the gen ned A, this enzyme releases sialic acids (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids for use as carbon and energy sources due to its hydrolytic activity.  
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The CDS  of this enzymes comes from Micromonospora viridifaciens and was optimized for its expression in E. coli BL21 cells. Encoded by the gen ned A, this enzyme releases sialic acids (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids for use as carbon and energy sources due to its hydrolytic activity.  
  
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Additionally, for the proper characterization of this part, the sequence of the sfGFP was added to the construct and complemented with a His-Tag to facilitate its purification.  
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Additionally, for the proper characterization of this part, the sequence of the of the  sfGFP was added to the construct.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:55, 17 October 2019


T7-LacO Promoter + strong RBS + sialidase + sfGFP + 6x his-tag + double terminator

This device uses Bba_K2406020, Bba_J34801 and Bba_0010 to regulate the expression of a super folding GFP (Bba_I746916). The main objective of creating this device is to facilitate the characterization of any coding sequence added next to the sfGFP. In our case, we decided to add the coding sequence of sialidase from M. viridifaciens.

The CDS of this enzymes comes from Micromonospora viridifaciens and was optimized for its expression in E. coli BL21 cells. Encoded by the gen ned A, this enzyme releases sialic acids (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids for use as carbon and energy sources due to its hydrolytic activity.

Additionally, for the proper characterization of this part, the sequence of the of the sfGFP was added to the construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1986
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 646
    Illegal BsaI.rc site found at 510
    Illegal BsaI.rc site found at 1647
    Illegal SapI.rc site found at 2055