Difference between revisions of "Part:BBa K2960011:Design"
Aemetzloff (Talk | contribs) (→Design Notes) |
|||
| Line 9: | Line 9: | ||
The mlrF gene sequence has been codon-optimized for expression in E. coli. | The mlrF gene sequence has been codon-optimized for expression in E. coli. | ||
| − | + | The construct was designed to be RFC[10] compatible. | |
===Source=== | ===Source=== | ||
Revision as of 01:29, 17 October 2019
The mlrF enzyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mlrF gene sequence has been codon-optimized for expression in E. coli.
The construct was designed to be RFC[10] compatible.
Source
Sphingopyxis sp. genomic sequence. Jin H, Nishizawa T, Guo Y, Nishizawa A, Park H-D, Kato H, Tsuji K, Harada K-I. 2018. Complete genome sequence of a microcystin-degrading bacterium, Sphingosinicella microcystinivorans strain B-9. Microbiol Resour Announc 7:e00898-18. https://doi.org/10.1128/MRA.00898-18