Difference between revisions of "Part:BBa K3292001:Design"
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<partinfo>BBa_K3292001 short</partinfo> | <partinfo>BBa_K3292001 short</partinfo> | ||
| − | <partinfo> | + | <partinfo>Bba_J34801 SequenceAndFeatures</partinfo> |
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Promoter Bba_K2406020 | Promoter Bba_K2406020 | ||
| − | RBS | + | RBS Bba_J34801 |
| − | Terminator | + | Terminator Bba_B0010 |
sfGFP BBa_I746916 | sfGFP BBa_I746916 | ||
The sialidase coding sequence was retrieved from UNIPROT (Q02834 (NANH_MICVI)) | The sialidase coding sequence was retrieved from UNIPROT (Q02834 (NANH_MICVI)) | ||
===References=== | ===References=== | ||
Latest revision as of 01:14, 17 October 2019
T7-LacO Promoter + strong RBS + sialidase + sfGFP + 6x his-tag + double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The purification of the whole construct presented some disadvantages when using imidazole.
Source
Promoter Bba_K2406020 RBS Bba_J34801 Terminator Bba_B0010 sfGFP BBa_I746916 The sialidase coding sequence was retrieved from UNIPROT (Q02834 (NANH_MICVI))