Difference between revisions of "Part:BBa K2558003:Experience"

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===Applications of BBa_K2558003===
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===dCas9 Fluorescence Loss Assays===
  
 
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Revision as of 22:07, 16 October 2019


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Please enter how you used this part and how it worked out.

dCas9 Fluorescence Loss Assays

Figure1: Fluorescence Loss Assay. Amount of GFP fluorescence under the induction of dCas9 targeting GFP. The expression of dCas9 is regulated by an IPTG inducible expression system. Sp1 - 3 represent three different spacers targeting GFP or the associated promoter. SpNT represents a non-targeting spacer acting as a control.

User Reviews

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