Difference between revisions of "Part:BBa K3176001"

Latest revision as of 21:30, 16 October 2019

mRFP for S.aureus with SarA RBS

mRFP1 is a constitutively monomeric red fluorescent protein derived from Discosoma coral drFP583 gene. It is widely used as a reporter because it matures 10 times faster than DsRed and shows a greater tissue penetration. BBa_K3176001 allows the expression of mRFP1 in gram-positive cells. It was designed from BBa_E1010 and adapted for S. aureus and can thus be used as a reporter for this strain by inserting the promoter SarA P1 from BBa_K1323021 and a SarA RBS from BBa_3176010. The excitation peak is at 584 nm and it shows an emission peak at 607 nm.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 623
Illegal AgeI site found at 735
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3176001 short</partinfo>
-mRFP1 is a constitutively monomeric red fluorescent protein derived from Discosoma coral drFP583 gene. It is widely used as a reporter because it matures 10 times faster than DsRed and shows a greater tissue penetration. BBa_K3176001 allows the expression of mRFP1 in gram-positive cells. It was designed from BBa_J04450 and adapted for S. aureus and can thus be used as a reporter for this strain. The excitation peak is at 584 nm and it shows an emission peak at 607 nm.
+mRFP1 is a constitutively monomeric red fluorescent protein derived from Discosoma coral drFP583 gene. It is widely used as a reporter because it matures 10 times faster than DsRed and shows a greater tissue penetration. BBa_K3176001 allows the expression of mRFP1 in gram-positive cells. It was designed from BBa_E1010 and adapted for S. aureus and can thus be used as a reporter for this strain by inserting the promoter SarA P1 from BBa_K1323021 and a SarA RBS from BBa_3176010. The excitation peak is at 584 nm and it shows an emission peak at 607 nm.
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