Difference between revisions of "Part:BBa K3032112"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K3032112 short</partinfo> | ||
+ | |||
+ | This is an Anderson (0.16) promoter with EL222 binding site inside of it. Promoter is designed in such a way that EL222 repressor (BBa_K2332004) could precisely bind to it. EL222 DNA binding sequence was inserted into weak promoter’s region of -35 to -10. | ||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K3032112 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K3032112 parameters</partinfo> | ||
+ | <!-- --> |
Latest revision as of 18:53, 16 October 2019
EL222 regulated promoter based on Anderson (0.16)
This is an Anderson (0.16) promoter with EL222 binding site inside of it. Promoter is designed in such a way that EL222 repressor (BBa_K2332004) could precisely bind to it. EL222 DNA binding sequence was inserted into weak promoter’s region of -35 to -10.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 31
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]