Difference between revisions of "Part:BBa K3032112:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K3032112 short</partinfo>
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<partinfo>BBa_K3032112 SequenceAndFeatures</partinfo>
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===Design Notes===
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EL222 DNA binding sequence was carefully inserted into weak promoter’s region of -35 to -10 in order not to affect promoter's activity.
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===Source===
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This promoter sequence is design by us. EL222 DNA binding sequence (DOI: 10.1093/nar/gkw548) was inserted into weak promoter’s region of -35 to -10.
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===References===

Latest revision as of 18:51, 16 October 2019


EL222 regulated promoter based on Anderson (0.16)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

EL222 DNA binding sequence was carefully inserted into weak promoter’s region of -35 to -10 in order not to affect promoter's activity.


Source

This promoter sequence is design by us. EL222 DNA binding sequence (DOI: 10.1093/nar/gkw548) was inserted into weak promoter’s region of -35 to -10.


References